Table 3. GM1 medium recipe.

Compound	Amount	Final concentration
SBase 10x	10 ml	1x
Glucose 10%	5 ml	0.5 %
Yeast extract 2%	5 ml	0.1 %
Casein hydrolysate 2%	2 ml	0.02 %
MgSO4 1 M	80 μl	0.8 mM
D/L tryptophan 1.25 mg/ml	2 ml	0.025 %
L-Methionine 10 mg/ml	200 μl	0.02 %
ddH2O top up to	100 ml