Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 18;44(1):1–12. doi: 10.14348/molcells.2020.0143

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Korean Society for Molecular and Cellular Biology. All rights reserved.

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

PMC Copyright notice

Fig. 5 — (A) The inhibitory effects of TDAG51 on the promoter activity of aP2-Luc induced by PPARγ-RXRα coexpression. Reporter plasmids (aP2-Luc [0.1 µg] and pcDNA3.1/His/LacZ [0.1 µg]) were cotransfected with epitope-tagged expression plasmids (Flag-PPARγ [0.25 µg], Myc-RXRα [0.1 µg], and/or Xp-TDAG51 [0.25-0.5 µg]) into 293T cells. The expression of transfected plasmids was analyzed by immunoblotting against anti-epitope antibodies. β-Actin was used as the loading control. (B) The inhibitory effects of TDAG51 on the promoter activity of aP2-Luc induced by PPARγ expression with rosiglitazone treatment. (C) The inhibitory effects of TDAG51 deletion mutants on the promoter activity of aP2-Luc induced by PPARγ-RXRα coexpression. Reporter plasmids were cotransfected with epitope-tagged expression plasmids (Flag-PPARγ [0.25 µg], Myc-RXRα [0.1 µg], and/or GST-TDAG51 [0.5 µg]) into 293T cells. GST alone (mock) was used as a control. **P < 0.01; ***P < 0.001; n.s., not significant.