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. 2019 Jun 5;9(11):e3253. doi: 10.21769/BioProtoc.3253

Figure 4. Calculated binding curves showing the relationship between [PL] and [Ltot] at a constant [Ptot].

Figure 4.

Here [Ptot] is chosen to be higher than the Kd’s but still in a reasonable range. A. Kd = 0.01 μM, Ptotal = 0.1 μM, Ltotal = 1 μM. B. Kd = 0.001 μM, Ptotal = 0.1 μM, Ltotal =1 μM.