Table 3. Inner filter effect data processing for haem.
| [Haem] μM | 0 | 0.3984 | 0.7936 | 1.3807 | 2.15264 | 3.68932 | 5.60928 | 7.52895 | 11.3462 | 17.0172 | 36.0687 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Fluorescence intensities at top 5 wavelengths | 720.154 | 697.296 | 656.790 | 644.240 | 619.960 | 576.750 | 536.299 | 495.699 | 428.407 | 350.978 | 178.493 |
| 731.785 | 695.609 | 662.792 | 642.030 | 613.975 | 576.054 | 536.828 | 494.665 | 432.185 | 354.062 | 179.209 | |
| 724.467 | 693.689 | 664.013 | 638.424 | 617.182 | 579.671 | 536.130 | 497.589 | 431.487 | 353.565 | 180.623 | |
| 718.830 | 691.224 | 659.906 | 642.552 | 612.479 | 576.233 | 531.706 | 489.314 | 428.199 | 350.630 | 179.322 | |
| 725.489 | 692.393 | 665.459 | 638.383 | 611.570 | 577.338 | 531.688 | 495.957 | 431.974 | 354.534 | 178.990 | |
| Average Intensity | 724.145 | 694.042 | 661.792 | 641.126 | 615.033 | 577.209 | 534.531 | 494.645 | 430.450 | 352.754 | 179.327 |
| Difference from expected Fl intensity | 0.000 | -30.103 | -62.353 | -83.019 | -109.112 | -146.936 | -189.615 | -229.500 | -293.695 | -371.392 | -544.818 |
| % difference from inner filter effect | 0.000 | -4.157 | -8.611 | -11.464 | -15.068 | -20.291 | -26.185 | -31.693 | -40.557 | -51.287 | -75.236 |
| Corrected Fl Intensity | 724.145 | 724.145 | 724.145 | 724.145 | 724.145 | 724.145 | 724.145 | 724.145 | 724.145 | 724.145 | 724.145 |
| Fluorescence scaled /1 | 1.000 | 0.958 | 0.914 | 0.885 | 0.849 | 0.797 | 0.738 | 0.683 | 0.594 | 0.487 | 0.248 |