Figure 1. Schematic and expected dispersion patterns of Bnl:HA₁GFP₃.

A. Schematic of the chimeric Bnl:HA₁GFP₃ protein, where an HA and GFP tag are located upstream and downstream of a Furin cleavage site, respectively. Bnl is cleaved at 164^th amino acid and the HA and GFP tags are located at the 87^th and 432^nd residues, respectively. B. Cartoons of wing discs expressing Bnl:HA₁GFP₃ (yellow: GFP + α-HA red immunostaining) in the Bnl source. In ex vivo culture conditions without Furin inhibitors (left wing disc), Bnl is cleaved and only the GFP-tagged fragment of Bnl is transported to the ASP recipient cells. In ex vivo conditions with Furin inhibitors included, the amount of full length Bnl:HA₁GFP₃ (yellow) puncta in the ASP gradually increases with longer incubation time (right wing disc).