Figure 2. Dissection scheme of a late 3^rd instar Drosophila Larva.

A 3-step dissection is shown for a late 3^rd instar larva. First, the posterior third of the larva is cut off and discarded, leaving just the desired anterior portion. Next, the anterior portion of the carcass is inverted using forceps to push the mouth hooks through the rest of the anterior portion, resulting in the externalization of all interior tissues including the wing imaginal discs. Finally, as much of the gut as possible is removed without disturbing the imaginal discs.