Figure 3. Harvesting of the tumor.

To harvest the tumors, the eggs are put on ice for at least 30 min. During this time, the windows are enlarged to enable a more precise handling (A). If desired, pictures of the tumors in the egg can now be taken. Labeling of the tumor cells with a fluorophore may help to identify the area of tumor if the scaffold is barely visible. Then the part of the CAM around the tumor is excised (B), sandwiched between two embedding sponges (C) and placed in an embedding mold (D). The samples are now ready to be fixed, plunged in paraffin and embedded.