Figure 3.

Expression of Anti-tau scFvs and iBs in the Midbrain and Spinal Cord Following Neonatal Intraspinal AAV Injection

JNPL3 mice were injected intraspinally at P0 with pAAV2/8 (2 × 10¹⁰ genomes) expressing PHF1, CP13, and Tau5 iBs and scFvs. Control mice were injected with rAAV-EGFP. (A) Widespread iB and scFv expression was visualized with an anti-c-myc antibody in the spinal cord on representative paraffin sections. Scale bar, 100 μm. (B) Relative iB and scFv levels in brain homogenates, spinal cord homogenates, and plasma of injected mice detected via sandwich ELISA. n = 4. Data represent mean ± SEM. ∗∗∗p < 0.001, by unpaired two-tailed Student’s t test.