Figure 5.

p-Tau-Specific iBs and scFvs Delay the Onset of Hindlimb Paralysis of Homozygous JNPL3 Mice

JNPL3 mice for the survival cohort were intraspinally injected at P0 with pAAV2/8 (2 × 10¹⁰ genomes) expressing PHF1, CP13, and Tau5 iBs or scFvs. Control mice were injected with rAAV-EGFP. All injected mice reached a terminal state (paralysis) within 12 months post-injection (n = 10–12). (A) Kaplan-Meier survival plots show an increased time to terminal state (killed due to paralysis) for mice expressing PHF1i, CP13i, and PHF1s. ∗∗p < 0.002, ∗∗∗∗p < 0.000002. (B) Median age of terminal state and relative increase of lifespan. (C) Mantel-Cox analysis of lifespan of mice overexpressing iBs and scFvs, all cohorts compared with each other, when multiple comparisons were taken into account. ∗p ≤ 0.008, ∗∗p ≤ 0.0002, ∗∗∗p ≤ 0.00002, ∗∗∗∗p ≤ 0.000002. ns, not significant (p > 0.008).