Table 1.
Sequences of scFvs and iBs
| Parent mAb | Amino Acid Sequence | |
|---|---|---|
| Variable heavy chain (VH) | CP13 parent | EVQLVETGGGLVQPKGSLKLSCAASGFTFNTSAMNWVRQAPGKGLEWVARIKSKSNKYTTSYADSVKD RFTISRDDSQSMLYLQMYNLKTEDTAMYYCMNSAMDYWGQGTSVTVSS |
| CP13 iB | EVQLVESGGGLVQPGGSLRLSCAASGFTFNTSAMNWVRQAPGKGLEWVSRIKSSSNKYTTSYADSVKG RFTISRDNSKNTLYLQMNSLRAEDTAVYYCMNSAMDYWGQGTLVTVSS |
|
| PHF1 parent | EVQLQQSGPELVKPGASVKISCKTSGYTFTEYTIHWVKQSHGESLEWIGGINPNDGGTIYNQKFKGKA TLTVDKSSKTAYMELRSLTSEDSAVFYCARGPSARFPYWGQGTLVTVSA |
|
| PHF1 iB | EVQLVQSGPGLVQPGGSLRLSCATSGYTFTEYTIHWVRQAPGESLEWVSGINPSDGGTIYNQSVKGRF TLSVDNSKNTAYLQMNSLRAEDTAVYYCARGPSARFPYWGQGTLVTVSS |
|
| Tau5 parent | EVQLQQSGAEIVRSGASVKLSCAASGFNIKDYYMHWVKQRPEQGLEWIGWIDPENGDIAYAPKFQGKA TMTADTSSNTAYLQLSRLTSEDTAVYFCNGRGGMITTDFFDYWGQGTTLTVSS |
|
| Tau5 iB | EVQLVQSGAGLVQPGGSLRLSCAASGFNIKDYYMHWVRQAPGQGLEWVSWIDPSNGDIAYAPSVQGRF TMSADNSKNTAYLQMNSLRAEDTAVYFCNGRGGMITTDFFDYWGQGTLVTVSS |
|
| Variable light chain (VL) | CP13 parent | EIVMTQAVSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPHLLIYRMSNLASGVPDRFSGSGS GTAFTLRISRVEAEDVGVYYCMQHLEYPLTFGAGTKLELKR |
| CP13 iB | EIVMTQSPATLSLSPGERATLSCRSSQSLLHSNGNTYLYWFQQRPGQSPRLLIYRMSNRASGVPARFSGS GSGTAFTLTISSLEPEDFAVYYCMQHLEYPLTFGQGTKVEIKR |
|
| PHF1 parent | DVVMTQTPLTLSVTIGQPASISCKSSQSLLDSDGKTYLNWLLQRPGQSPKRLIYLVSKLDSGVPDRFTGS GSGTDFTLKISRVEAEDLGVYYCWQGTHFPRTFGGGTKLEIKR |
|
| PHF1 iB | DVVMTQSPATLSLSPGERATLSCRSSQSLLDSDGKTYLNWLQQRPGQSPRRLIYLVSKRDSGVPARFTG SGSGTDFTLTISSLEPEDFAVYYCWQGTHFPRTFGQGTKVEIKR |
|
| Tau5 parent | DVLMTQTP–LSLPVSLGDQASISCRSSQSIVHSNGNTYLEWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGS GSGTDFTLKISRVEAEDLGVYYCFQGSHVPWTFGGGTKLEIKR |
|
| Tau5 iB | DVVMTQSPATLSL–SPGERATLSCRSSQSIVHSNGNTYLEWYQQKPGQSPRLLIYKVSNRFSGVPARFSGSG SGTDFTLTISSLEPEDFAVYYCFQGSHVPWTFGQGTKVEIKR |
Original sequences of variable regions from PHF1, CP13. and tau5 mAbs and modified sequences of iBs. Mutations in the VH and VL chains, which were made to promote stability of intracellularly expressed scFvs,49 are shown in bold.