Figure 4.

Off-Target Effect Evaluation upon 2′-MOE Gapmer Treatment

Immortalized patient-derived myotubes transfected with 100 nM MOE1, MOE2, or MOE3 (numbered) at 13 days post-differentiation were harvested a day later and used for quantitative real-time RT-PCR expression analysis of (A) BANF1, (B) SSR4, (C) FARP1, and (D) ZBTB7B. Error bars: SD; n = 3 independent experiments. ∗p < 0.05, ∗∗p < 0.005 versus NT, one-way ANOVA with Dunnett’s test. ^δp < 0.05, unpaired, two-tailed t test.