Figure 2.

A Single-Cell Atlas of CAR-T and Endogenous T Cells during CAR-T Therapy

(A) Overall study design of single-cell RNA-seq. Single-cell RNA-seq is applied to CAR-T (CD3⁺CAR⁺) cells and endogenous T (CD3⁺CAR⁻) cells derived from PBMCs. (B) The UMAP visualization of 55,488 cells from CAR-T and endogenous T cell samples at three phases. 24 clusters are indicated by different colors. Sample origins are distinguished by colors as shown at top-left corner. (C) Expression patterns of CD4, CD8A, CD25, and GZMB are exhibited on UMAP plot. Color intensity represents expression level. (D) Proportions of CD4⁺ T cells and CD8⁺ T cells within each sequenced sample. Only cells expressing CD4 or CD8 are taken into account.