Figure 2.

The Cytolytic Effects of oHSV

(A) Pan02 (open bar) and Pan02_HVEM (closed bar) cells were mock infected or infected with HSV-1 or oHSV at MOI = 1 for 24 h, stained with APC-conjugated Annexin V, and then subjected to flow cytometry analysis. The percentages of apoptotic cells (Annexin V⁺) were plotted in columns. (B) Pan02_HVEM cells were mock infected (open circles) or infected with HSV-1 (open squares) or oHSV (closed circles) at MOI = 1, and cell proliferation was monitored by a CCK8 assay. The absorbance at wavelength of 450 nm was determined and plotted against time. Bracket, differences between mock-treated and oHSV-treated groups were statistically analyzed by two-tailed Student’s t test. (C) Pan02 (top panels) and Pan02_HVEM (bottom panels) cells were mock infected or infected with indicated viruses at MOI = 1, and CPE on cells were observed at 24 hpi. Scale bar, 100 μm. The results were representative for three independent experiments. The data were expressed as the mean values with standard deviation (n = 3). Statistics analysis was performed using two-tailed Student’s t test. n.s., not significant. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.