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. 2020 Oct 22;29(2):718–733. doi: 10.1016/j.ymthe.2020.10.019

Figure 2.

Figure 2

K3 and K5 Downregulate T Cell Surface Expression of MHC

K3.myc-Qstalk (K3)-, K5.myc-Qstalk (K5)-, and mock (retroviral vector encoding Qstalk marker)-transduced T cells were selected by magnetic sorting. Flow cytometry analysis to determine nontransduced (NT)-, K3-, K5-, or mock-modified T cell-surface expression of MHC class I molecules (A) by HLA-ABC clone w6/32 antibody, (B) or by human β2-microglobulin antibody. (C) MHC class II surface expression was measured with antibody against HLA-DR. Representative flow plots from two donors are shown (left). n = 4. Paired t test was used to compare indicated groups. ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; NS, not significant.