Skip to main content
. 2020 Oct 22;29(2):718–733. doi: 10.1016/j.ymthe.2020.10.019

Figure 3.

Figure 3

K3 and K5 Protect T Cells from Peptide-Specific TCR-Directed Cytotoxicity

(A) Flow cytometry analysis to determine the surface expression of NLV peptide-specific CMV-156 TCR on HLA-A2− donor T cells (n = 3), 14 days post-transduction. (B–D) K3-, K5-, or mock (Qstalk alone)-modified HLA-A2+ donor T cells, labeled with CellTracker Green CMFDA dye and pulsed with either NLV or SLL (10 μg/mL) peptides, were cocultured with HLA-A2− donor T cells (n = 3) for 24 h at different E:T ratios. Cells were stained for viability v450, anti-HLA-A2, and subjected to flow analysis. NLV- or SLL-pulsed T cells were first gated as CMFDA+ populations. Dead cell populations were further gated as viability dye-positive cells. Two-way ANOVA was used to determine the differences among groups. For NLV-pulsed T cell killing (C), p < 0.0001; for SLL-pulsed T cell killing (D), not significant.