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. 2021 Jan 20;11:622153. doi: 10.3389/fphar.2020.622153

FIGURE 5.

FIGURE 5

TET2 is required for empagliflozin-induced autophagy. HL7702 cells were transfected with or without TET2 siRNA and treated with or without PA and HG, with or without empagliflozin (A) Representative images of Oil Red O staining and (B) quantitative analysis of TGs. Scale bar represents 30 μm. Immunofluorescence of (C) TET2 and (D) p62. Scale bars represent 30 μm (C) and 100 μm (D), respectively (E) Western blot analyses of TET2, ATG5, Beclin-1, and p62 with β-actin as a loading control and (F–I) densitometric analyses of band intensities normalized to β-actin. Data in (B) and (F–I) are presented as means ± SEM from three independent experiments. Empa, empagliflozin; HG, high glucose; NC, negative control group; PA, palmitic acid; TG, triglyceride. *p < 0.05, **p < 0.01, ***p < 0.001.