Fig. 4.

ADMA positively regulated β-catenin expression in GC and mediated through WNT signaling pathway. a Western blot demonstrated that ADMA (10 μM) enhanced the protein levels of β-catenin in AGS and MGC803. β-actin served as a loading control. b The ADMA(10 μM) in GC cell positively correlated with β-catenin activity in TOP-Flash reporter assay. Expression was normalized with Renilla luciferase activity. The experiments were performed three times independently(*P < 0.05). c IHC assay showing the relationship between serum ADMA level and β-catenin in the 115 GC samples. Scale bar, 50 μm. In the GC samples with high ADMA level, the percentage of β-catenin positive expression was 64%, significantly higher than those with low ADMA level (22.9%) (*P < 0.05). d The stimulatory effect of ADMA on the GC cell migration and invasion blocked by WTN inhibitor XAV939, as the representative images were presented (*P < 0.05; NS, no statistical significance)