Fig. 4.

Scheme on generating Tie2-CreERT2 transgenic rat. a Genomic structure after CreERT2-mCherry insertion at the Tie2 locus using CRIPSR/Cas9 approach. Genotyping primers are labeled in red. b Genotyping results of potential founders using PCR primers amplifying internal, 5′ junction and 3′ junction for transgene insertion. #3 animal is confirmed to be a positive transgenic founder. c–e Genotyping results of F1 progeny of the # 3 founder using PCR primers amplifying internal (c), 5′ junction (d), and 3′ junction (e) for correct transgene insertion