Fig. 8.

Testing of tissue-specific Cre recombination using reporter line #21 (CAG-lox-Stop-lox-GFP-lacZ). Mor23-Cre/CAG-lox-Stop-lox-GFP-LacZ double transgenic rats were obtained by crossing the two single transgenic line (a). Multiple F1 animals containing both transgenes were identified by PCR primers amplifying internal fragments for Mor23-Cre or GFP-lacZ, respectively (b). Positive animals are in red No. (c) Brain sections of Mor23-Cre/CAG-GFP-lacZ double positive animals were examined for GFP expression by green fluorescence (b) or Cre expression (c) by antibody staining using anti-Cre antibody