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. Author manuscript; available in PMC: 2021 Nov 15.
Published in final edited form as: Int J Pharm. 2020 Sep 2;589:119843. doi: 10.1016/j.ijpharm.2020.119843

Figure 4. Thermostability of liquid or lyophilized Pfs25 SNAP vaccine.

Figure 4.

A-D) Accelerated thermal degradation of the liposomes lyophilized and liquid formula stored at 4, 40 and 60 °C for 2 weeks A) Photo of liquid lyophilized formula after reconstitution B) UV-VIS spectrum. C) LC/MS analysis of lipid content D) Pfs25 binding to the liposomes determined by microcentrifugal filtration, E-I) Forced thermal degradation of Pfs25 stored at 4, 40 and 60 °C for 2 weeks E) Total protein content after incubation determined by the BCA assay. F) Free amino groups determined by the OPA assay. G) Sandwich ELISA of the pre-incubated proteins, mAb 1245 was used as a capture antibody while mAb 1269 was used as the detection antibody. H) SDS-PAGE of Pfs25 after storage at various temperatures. I) SDS-PAGE after immune precipitations using anti-Pfs25 monoclonal antibodies using either mAb 1245 or mAb 1269 to capture Pfs25 after incubation at different temperatures for two weeks, the arrow indicates Pfs25 band while the other two bands are the heavy and the light chains of the mAbs. The first lane contains 1μg of Pfs25, second and sixth lane contain only mAbs. Bar graphs in C-G show mean ± s.d. for n = 3.