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. Author manuscript; available in PMC: 2021 Nov 15.
Published in final edited form as: Int J Pharm. 2020 Sep 2;589:119843. doi: 10.1016/j.ijpharm.2020.119843

Figure 5. Immunogenicity of lyophilized and liquid Pfs25-bound CoPoP liposomes following storage at elevated temperatures.

Figure 5.

Mice were immunized with 100 ng Pfs25 that was first complexed with CoPoP liposomes and stored at the indicated temperatures and durations. A) Anti-Pfs25 IgG ELISA. Statistical analysis based on one-way ANOVA followed by Tukey test, using log transformed data (***P<0.001). Lines represent geometric mean. B) Functional testing in the SMFA. The inhibition activity statistical analysis is based on a zero-inflated negative binomial random effects model and asterisks show significant inhibition compared to the post-immune IgGs from mice immunized with CoPoP liposome without Pfs25. ***P<0.001 and *P < 0.05. Lines show upper and lower 95% confidence intervals. Negative values are assigned as zero for presentation purpose.