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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Biochim Biophys Acta Gene Regul Mech. 2020 Dec 16;1864(2):194669. doi: 10.1016/j.bbagrm.2020.194669

Figure 2.

Figure 2.

Identification of the SAGA and SLIK/SALSA complexes. Schematic representation of the original isolation of SAGA and SLIK/SALSA from yeast whole cell extracts, adapted from [4, 5]. Lower panel depicts fluorograms of HAT assays performed with nucleosomal histones and Mono Q fractions, which helped isolate the SAGA, SLIK, ADA, NuA3 and NuA4 HAT complexes. SLIK/SALSA was independently purified by Sterner et al, 2002 from synthetic complete media under conditions where it preferentially bound Nickel-nitrilotriacetic acid (NTA) agarose.