Table 1. General health profile of AKT-2 HET and KO mice.

Physical characteristics: body weight, poor coat condition, bald patches, missing whiskers, pilorerection, body tone, limb tone and physical abnormalities. Motoric abilities: trunk curl, forepaw reaching (*p ≤ 0.05, ***p ≤ 0.001 vs WT), wire hang, positional passivity (**p ≤ 0.05, *p ≤ 0. 1 vs WT). Reflexes: righting, corneal, ear twitch, reactivity to handling and petting escape. Empty cage behavior: transfer freezing, wild running, exploration, grooming time and events, rearing events (***p ≤ 0.001 vs WT) and digging events. Values represent percentage or mean ± SEM. N = 11 WT N = 12 HET, N = 6 KO.

General Health	WT	HET	KO
Physical characteristics
Body weight (g)	26.94±0.61	26.79±0.50	25.48±0.59
Poor coat condition (%)	0	0	0
Bald Patches (%)	0	0	0
Missing whiskers (%)	0	0	0
Piloerection (%)	0	0	0
Body tone (% of good)	100	100	100
Limb tone (% of good)	100	100	100
Physical abnormalities (%)	0	0	0
Motoric abilities
Trunk curl (%)	91	83	83
Forepaw reaching (%)	91	25***	33*
Wire hang (sec)	60.0±0.0	60.0±0.0	60.0±0.0
Positional passivity (%)	0	42**	33*
Reflexes (% of mice normal)
Righting reflex (%)	100	100	100
Corneal (%)	100	100	100
Ear twitch (%)	100	100	100
Whisker twitch (%)	100	100	100
Reactivity to handling (3-point scale)	2.04±0.04	2.15±0.09	2.25±0.14
Petting escape (%)	36	46	25
Empty cage behavior
Transfer freezing (%)	0	0	0
Wild running (%)	0	0	0
Exploration (3-point scale)	2.36±0.15	2.33±0.14	2.33±0.21
Grooming (sec)	8.06±1.11	6.40±1.10	8.41±2.23
Grooming (events)	5.91±0.94	4.67±0.61	4.33±0.61
Rearing (events)	33.55±1.64	50.17±2.58***	54.17±2.23***
Digging (events)	10.18±2.14	13.83±2.30	13.00±2.28