Figure 6.

Schematic representation of the bioprinting process and features of a multi-cellular GBM model. a) GSCs, macrophages, astrocytes, and NPCs were bioprinted in an HA-GelMA hydrogel with spatial separations. b) GSCs in 3D-bioprinted model recapitulated transcriptional profiles of tumor tissue. c,d) 3D TME with macrophages promoted invasiveness and drug resistance of the GSCs. e) 3D TME differentially polarized monocytes to M2 macrophage phenotype. f,g) Novel functional dependencies indicated by 3D-bioprinted models were validated both in vitro and in animal models. Reproduced with permission. Copyright Springer Nature, 2020.^[9] Reproduced with permission.