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. Author manuscript; available in PMC: 2021 Jun 23.
Published in final edited form as: Nat Neurosci. 2020 Dec 23;24(2):204–213. doi: 10.1038/s41593-020-00762-9

Extended Data Fig. 7. ISR activation of E18.5 PBS and MIA male fetus in SFB-negative dams.

Extended Data Fig. 7

Immunoblot analysis measuring phospho-eIF2α and quantification in E18.5 PBS and MIA male fetal cortices, either from SFB-positive or SFB-negative B6 dams. SFB-positive dams were obtained from Taconic Biosciences (Tac) whereas SFB-negative dams from Jackson laboratory (Jax) and maintained in SFB-positive and negative conditions, respectively. Y-axis represents relative blot intensity to each PBS control (Two-tailed unpaired t-test: Tac t = 4.659, df = 6, **P = 0.0035; Jax t = 0.9013, df = 6; n = 4 pups from 2 litters). Data are mean ± SEM; see Supplementary Table 20 for detailed statistics. Unprocessed blots are provided as a Source Data file.