Fig. 2 |. RORB-expressing excitatory neuron subpopulations in the EC are selectively vulnerable.

a-b, tSNE projection of excitatory neurons from the EC (a) and SFG (b) in their respective alignment spaces, colored by individual of origin (center) or subpopulation identity (outer). c-d, Heatmap and hierarchical clustering of subpopulations and subpopulation marker expression (top subpanel); “High” and “Low” relative expression reflect above- and below-average expression, respectively (see Methods). Relative abundance of subpopulations across Braak stages (second subpanel); for each brain region, statistical significance of differences in relative abundance across Braak stages (Braak 0 n=3, Braak 2 n=4, Braak 6 n=3, where n is the number of individuals sampled) was determined by beta regression and adjusted for multiple comparisons (see Methods). Expression heatmap of EC layer-specific genes identified from Ramsden et al.²² (c, third subpanel). Expression heatmap of neocortical layer-specific genes from Lake et al.¹² (d, third subpanel). Expression of selectively vulnerable subpopulation markers identified in the EC (bottom subpanel). e, Heatmap of Pearson correlation between the gene expression profiles of EC and SFG subpopulations.