Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 2;12:751. doi: 10.1038/s41467-021-21025-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a Verification of shRNA. HeLa cells were transfected with control shRNA (shCtrl) or shRNA against Cdc42 (shCdc42), coexpressed with wild-type Clover-Cdc42 or its shRNA-resistant mutant (Clover-Cdc42res), respectively. The transfection of shCdc42 suppresses the expression of Clover-Cdc42 (lane 2 in the top panel). The transfection of shCdc42 does not suppress the expression of Clover-Cdc42res (lane 3 in the top panel). Averaged time courses of spine volume change upon single-spine stimulation (b) or clustered-spine stimulation (e) in neurons under manipulations of Cdc42 signaling. For single-spine stimulation, n(spines/neurons) = 17/6 shCtrl, 13/7 shCdc42, and 12/4 rescue. For clustered-spine stimulation, n(spines/neurons) = 51/6 shCtrl, 45/7 shCdc42, and 25/4 rescue. Quantification of transient (c, averaged over 4–6 min) and sustained (d, averaged over 20–30 min) spine volume change after single-spine stimulation. The data set used in b was analyzed. n(spines/neurons) is the same as in b. f Quantification of spine volume change (averaged over 20–30 min) after clustered-spine stimulation. The data set used in e was analyzed. n(spines/neurons) is the same as in e. g A model of CaMKII-dependent Cdc42 activation and its relationship with sLTP. Simultaneous activation of CaMKII in clustered spines leads to enhanced Cdc42 activity and sLTP. For all figures, the data are presented as mean ± SEM. Statistical comparisons were performed using two-tailed unpaired t test (c, d, and f). ***p < 0.001; **p < 0.01; *p < 0.05; N.S. not significant. Source data are provided as a Source Data file.