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. 2021 Feb 2;11:2784. doi: 10.1038/s41598-021-82431-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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E.C.M. detachment induces EZH2 methyltransferase expression and activity. (a) All three endogenous cancer cells were grown in an ultra-low attachment plate for 30 min, 6 h, and 24-h. After a specific time point, R.N.A. was isolated, and quantitative-PCR was performed for targeted genes, values were normalized with housekeeping gene RPLP0. Error bars illustrate mean ± S.E.M. (b) H3K27 mono, di, and trimethylation activity assay of the nuclear lysates of HCT116 and HeLa during E.C.M. detached vs. attached condition.