Figure 3.

Cell clustering is critical for EZH2 induction during matrix detachment. All three endogenous cancer cells were grown in ultra-low attachment plates both in the presence and absence of EDTA for 24-h. Furthermore, after completion of the time point, R.N.A. was isolated, and quantitative-PCR was performed for targeted genes, values were normalized with housekeeping gene RPLP0. Bars depicting normalized data (mean ± S.E.M., n = 3), *P < 0.05.