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. 2021 Feb 2;11:2808. doi: 10.1038/s41598-020-80529-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro cytotoxicity analysis of gold nanoformulations against PC3 cell lines at different time intervals such as 24 h, 48 h, and 72 h using premix WST cell proliferation assay kit. (a) AuNPs, (b) PEG-AuNPs, (c) Dtx, (d) FA (e) AuNPs nanoformulations and (f,g) Comparison between Dtx, FA and nanoformulations at 24 and 48 h post treatment, and (h) PC3 cell images at control and nanoformulations. [*Corresponds to the statistical significance values (p < 0.05)].