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. 2021 Feb 2;7:8. doi: 10.1038/s41421-020-00238-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Unsupervised hierarchical clustering (UHC) with all expressed genes (301 cells, 13,227 genes) and a heatmap of the levels of selected marker genes. Color bars under the dendrogram indicate embryonic days (top) and cell types (bottom), respectively. b Violin plots of the expression of selected lineage specifier genes. c The development trajectory of each lineage based on psudotime analysis using Monocle2. d Cell types defined in the developmental trajectory according to the different branches of TE cells in c. e Expression patterns of mural TE- and polar TE-related genes in type 2 and 3 TEs. Stage information was indicated as follows: Pre-, Day 5 or/and Day 7; PreE-, Day 5; PreL-, Day 7; Post-, Day 10 and Day 12; PostE-, Day 10; PostL-, Day 12.