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. 2021 Feb 2;12:736. doi: 10.1038/s41467-021-20998-8

Table 1.

KI values (with standard deviations) for PARPi binding to PARP1 and PARP2 alone or in the presence of HPF1.

PARP1 PARP2
PARPi Structure Ki (nM) Ki + HPF1 (nM) Fold
(t-test)		 Ki (nM) Ki + HPF1
(nM)		 Fold
Olaparib graphic file with name 41467_2021_20998_Figa_HTML.gif

0.97 ± 0.17

(n = 6)

0.20 ± 0.01

(n = 4)

4.8

***

0.34 ± 0.06

(n = 4)

0.39 ± 0.10

(n = 4)

0.88

n.s.
AZD-2461 graphic file with name 41467_2021_20998_Figb_HTML.gif

2.2 ± 0.6

(n = 6)

0.78 ± 0.14

(n = 4)

2.9

**

 n.d. n.d
A-966492 graphic file with name 41467_2021_20998_Figc_HTML.gif

0.69 ± 0.12

(n = 3)

0.32 ± 0.05

(n = 3)

2.2

**

 n.d. n.d.
Niraparib graphic file with name 41467_2021_20998_Figd_HTML.gif

1.2 ± 0.4

(n = 5)

0.64 ± 0.03

(n = 3)

1.8

**

 n.d. n.d.
Veliparib graphic file with name 41467_2021_20998_Fige_HTML.gif

0.96 ± 0.08

(n = 5)

0.78 ± 0.07

(n = 4)

1.2

n.s.

9.9 ± 2.8

(n = 4)

2.9 ± 2.5

(n = 4)

1.7

n.s.
Rucaparib graphic file with name 41467_2021_20998_Figf_HTML.gif

0.09 ± 0.04

(n = 6)

0.07 ± 0.02

(n = 5)

1.4

n.s.

 n.d. n.d.
Talazoparib graphic file with name 41467_2021_20998_Figg_HTML.gif

0.012 ± 0.003

(n = 4)

0.011 ± 0.005

(n = 4)

1.1

n.s.

0.18 ± 0.04

(n = 3)

0.15 ± 0.03

(n = 3)

1.2

n.s.

All values were determined from replots as shown in Fig. 2. The number of replicates for each experiment is indicated, as well as the fold-difference caused by the addition of HPF1. Pairwise comparisons were evaluated using the two-sided student t test, as indicated by the asterisks: **P < 0.01, ***P < 0.001.