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. 2021 Feb 2;12:736. doi: 10.1038/s41467-021-20998-8

Table 2.

Kinetic and thermodynamic parameters for the binding of fl-Ola to PARP1 and PARP2.

PARP1 PARP2
kon (µM−1s−1) koff (hr−1) KD (pM) kon (µM−1s−1) koff (hr−1) KD (pM)
Nuc165

2.2 ± 0.6

n = 8

0.27 ± 0.02

n = 5

 35 ± 11

5.7 ± 1.1

n = 4

0.37 ± 0.06

n = 4

 18 ± 4.6
Nuc165/HPF1

4.4 ± 1.1

n = 7

0.12 ± 0.04

n = 5

 7.9 ± 3.1

2.3 ± 0.2

n = 4

0.21 ± 0.03

n = 3

 24.9 ± 4.7
p18mer

1.3 ± 0.3

n = 8

0.32 ± 0.03

n = 5

 70 ± 18

8.6 ± 2.3

n = 3

0.44 ± 0.04

n = 3

 14.1 ± 3.9
p18mer/HPF1

2.0 ± 0.4

n = 7

0.17 ± 0.03

n = 5

 24 ± 6

5.5 ± 0.8

n = 4

0.41 ± 0.08

n = 4

 20.5 ± 4.8

The values and standard deviations shown are from replicates (n) for each condition performed in duplicate.