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. 2021 Feb 2;12:750. doi: 10.1038/s41467-020-20289-8

Fig. 1. TGFβ signaling pathway remains active during myoblast differentiation.

Fig. 1

a qRT-PCR analysis of Tgfb1, 2, and 3 transcripts expression during in vitro differentiation of primary muscle cells shows different profiles. N = 3 biologically independent experiments for each time point. b qRT-PCR analysis of Alk5 and Tgfbr2 transcript expressions describes a constant expression of the receptors during primary muscle cell differentiation. N = 6 biologically independent experiments for each time point. c qRT-PCR analysis of the TGFβ target gene Smad7 transcript expression reveals a decreased activity of the pathway alongside in vitro primary muscle cell differentiation. N = 3 biologically independent experiments for each time point. d p-SMAD2/3 immunofluorescent staining of proliferating, differentiating, and differentiated primary myoblasts reveals a constant and basal activation of the pathway. N = 3 primary cell cultures. e p-SMAD2/3 and SMAD2/3 western-blot analysis of proliferating, differentiating, and differentiated primary myoblasts confirms a decrease in SMAD2/3 phosphorylation during differentiation. N = 3 biologically independent experiments. Scale bars: d 200 μm. Data are presented as mean ± SEM. Source data are provided as a Source Data file.