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. 2021 Feb 2;12:750. doi: 10.1038/s41467-020-20289-8

Fig. 2. The state of TGFβ signaling during in vivo muscle regeneration.

Fig. 2

a–c p-SMAD3, PAX7, and DYSTROPHIN immunofluorescent stainings on 0-, 4-, and 7-day post injury (d.p.i.) regenerating TA muscle cryosections. SMAD3 signaling is active in interstitial cells and PAX7+ cells (white arrows) during muscle regeneration. p-SMAD3 is strongly expressed by myonuclei of the regenerating myofibers marked by DYSTROPHIN at 4 d.p.i. N = 8 cryosections. d p-SMAD3, MYOGENIN, and DYSTROPHIN immunofluorescent staining on 4 d.p.i. regenerating TA muscle cryosections. Myocytes do not express p-SMAD3 at the time of differentiation. N = 8 cryosections. Scale bars: low magnification, 400 μm; high magnification, 100 μm.