| Antibodies |
| Rb pAb to MAX |
Abcam |
ab101271 |
| Rb mAb to c-Myc |
Abcam |
ab32072 |
| Rb mAB to DYKDDDDK Tag (D6W5B) |
Cell Signaling Technology |
Cat#14793S |
| Vinculin Rabbit Ab |
Cell Signaling Technology |
Cat#4650S |
| Rb mAb to c-Jun (60A8) |
Cell Signaling Technology |
Cat#9165S |
| Rb CDC25A Ab |
Cell Signaling Technology |
Cat#3652S |
| Rb mAb to CDK2 (78B2) |
Cell Signaling Technology |
Cat#2546S |
| Rb Anti-MAX |
Sigma-Aldrich |
HPA003474-100uL |
| GAPDH Ms McAb |
Proteintech |
Cat#60004-I-Ig |
| IRDye 680RD Goat anti-Mouse |
LI-COR |
926-60870 |
| IRDye 800CW Goat anti-Rabbit |
LI-COR |
926-32211 |
| B-Actin Rabbit mAb |
Cell Signaling Technology |
Cat#3700 |
| Bacterial and Virus Strains |
| One Shot Stbl3 Chemically Competent E. coli
|
Invitrogen |
Cat#C7373-03 |
| Biological Samples |
| N/A |
|
|
| Chemicals, Peptides, and Recombinant Proteins |
| c-MYC human recombinant protein |
Origene |
Sku#TP301611 |
| MAX human recombinant protein |
Origene |
AR09449PU-N |
| c-Jun human recombinant protein |
Origene |
AR09900PU-S |
| Invitrogen Hoechst 33342 dye |
Fisher Scientific |
Cat#H3570 |
| Dimethyl Sulfoxide |
Fisher Chemical |
D128-500 (CAS 67-68-5) |
| Dulbecco’s Phosphate Buffered Saline (DPBS) |
Sigma-Aldrich |
D8537-500mL |
| c-MYC human recombinant protein |
Abcam |
Ab169901 |
| MAX human recombinant protein |
Origene |
Sku#TP306812 |
| Opti-MEM Reduced Serum Media |
ThermoFisher Scientific |
Cat#31985062 |
| Lipofectamine 2000 Transfection Reagent |
ThermoFisher Scientific |
Cat#11668027 |
| Iodoacetamide 98% |
ACROS Organics |
Cat# AC122270050 |
| Iodoacetamide-13C2, 2-d2
|
Sigma-Aldrich |
Cat#721328 |
| Urea |
Fisher Chemical |
U15-500 (CAS 57-13-6) |
| ProteaseMAX Surfactant, lyophilized |
Promega |
REF#V2072 |
| Ammonium Bicarbonate |
Fisher Chemical |
A643-500 (CAS 1066-33-7) |
| TCEP 99% |
Strem Chemicals |
Cat#15-7400 (CAS 51805-45-9) |
| Formic Acid, 99.0+%, Optima LC/MS Grade |
Fisher Chemical |
A117-50 (CAS 64-18-6) |
| Sequencing Grade Modified Trypsin, Porcine |
Promega |
V511A |
| Covalent Ligand Library |
Enamine |
N/A |
| TAMRA-PEG4-Azide |
Click Chemistry Tools, Inc. |
AZ109-5 |
| Laemmli SDS sample buffer, reducing (4x) |
Alfa Aesar |
J60015 |
| Dharmafect I |
Dharmacon |
T-2001-02 |
| Anti-DYKDDDDK G1 Affinity Resin |
GenScript |
Cat#L00432 |
| 3X (DYKDDDDK) Peptide |
APExBIO |
A6001 |
| Tris(benzyltriazole methylamine) (TBTA) |
Sigma-Aldrich |
678937 (CAS 510758-28-8) |
| Biotin-TEV-azide |
Weerapana et. al., 2010 |
N/A |
| Copper (II) Sulfate |
Sigma-Aldrich |
451657 |
| AcTEV Protease |
Invitrogen |
12575-015 |
| N-Hex-5-ynyl-2-iodo-acetamide (IAyne) |
Chess Gmbh |
3187 |
| Spectra Multicolor Broad Range Protein Ladder |
Thermo Scientific |
Prod#26634 |
| Pierce Protease inhibitor Mini Tablets, EDTA-free |
Thermo Scientific |
Prod#A32955 |
| 5-TMRIA [Tetramethylrhodamine-5-Iodoacetamide Dihydroiodide] |
Setareh Biotech |
6222 |
| High Capacity Streptavidin Agarose Resin |
Thermo Scientific |
Ref#20357 |
| Streptavidin Agarose Resin |
Thermo Scientific |
Ref#20349 |
| Critical Commercial Assays |
| Abcam c-Myc Transcription Factor Assay Kit |
Abcam |
Ab207200 |
| MYC Luciferase Reporter |
Qiagen |
CCS-012L |
| Q5 site-directed mutagenesis kit |
New England Biolabs |
E0554S |
| Dual-Glo Luciferase |
Promega |
E2920 |
| Deposited Data |
| Experimental Models: Cell Lines |
| 231MFP |
(Jessani et al., 2004) |
N/A |
| HEK293T/17 |
ATCC |
CRL-11268 |
| MCF10A |
ATCC |
CRL-10317 |
| Experimental Models: Organisms/Strains |
|
Mouse model-- C.B-Igh-1b/IcrTac-Prkdcscid female mice |
Taconic |
CB17SC-F |
| Oligonucleotides |
| Primer: MYC C171Y forward ACT CCA CCT CCA GCT TGT ACC T |
This paper |
N/A |
| Primer: MYC C171Y reverse AGA CGC TGT GGC CGC |
This paper |
N/A |
| SASI_HS01_0015-0279/JUN (siJUN) 5’-CCUUCUAUGACGAUGCCCU[dt][dt]-3’ (sense) 5’- AGGGCAUCGUCAUAGAAGG-3’ (antisense) |
Sigma-Aldrich |
PDSIRNA2D SASI_HS01_0015-0279/ |
| siRNA Fluorescent Universal Negative Control#1, 6-FAM |
Sigma-Aldrich |
SIC007 |
| Recombinant DNA |
| Plasmid: pCMV6-Entry-cMYC (tag: c-term Myc-DDK) |
Origene |
RC201611 |
| Plasmid: pCMV6-Entry-cMYC (C171Y mutant, tag: c-term Myc-DDK) |
This study |
N/A |
| Plasmid: pCMV6-Entry-eGFP (tag: c-term Myc-DDK) |
(Ward et al., 2019) |
N/A |
| Software and Algorithms |
| ImageJ |
(Schneider et al., 2012) |
https://imagej.nih.gov/ij/ |
| IP2 proteomics pipeline 5.0.1 |
Integrated Proteomics Applications |
N/A |
| Other |
|
|
|
