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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: J Allergy Clin Immunol. 2020 Jul 21;147(2):470–483. doi: 10.1016/j.jaci.2020.04.063

Figure 4. Identification of polyclonal human Tfh13 cells.

Figure 4.

(A) Gating strategy for polyclonal human Tfh13 cells from an aeroallergen-sensitized patient after PMA/ionomycin stimulation. (B) IL-4 and IL-13 fluorescence minus one (FMO) and isotype control staining in cells from an allergic patient with stimulation. Pre-gated on circulating Tfh cells (live CD4+ CD3+ CD45RA– CXCR5+). (C) IL-4 and IL-13 intracellular staining in an allergic patient and a healthy control following no stimulation or PMA/ionomycin stimulation. Pre-gated on circulating Tfh cells. (D) IFN-γ and IL-13 intracellular staining in an allergic patient following PMA/ionomycin stimulation. Pre-gated on circulating Tfh cells. Samples were acquired on a BD FACSymphony A5 (BD Biosciences).