. Author manuscript; available in PMC: 2022 Feb 1.

Published in final edited form as: J Allergy Clin Immunol. 2020 Jul 21;147(2):470–483. doi: 10.1016/j.jaci.2020.04.063

Table 1.

Antibody panel for polyclonal murine Tfh13 cells, without IL-5

Step	Antigen (Clone)	Fluorophore	Dilution^*	Source
Surface	LIVE/DEAD™ Fixable Aqua Dead Cell Stain	(AmCyan channel)	1:1000 (1:500)	Life Technologies
Surface	PD-1 (RMP1–30)	Alexa Fluor® 647	1:200 (1:100)	BioLegend
Post-fixation 1°	CD4 (RM4–5)	APC-Fire™ 750	1:400	BioLegend
	CD44 (IM7)	Brilliant Violet 605™	1:600	BioLegend
	TCRβ (H57–597)	PerCP/Cyanine5.5	1:300	BioLegend
	IL-4 (11B11)	PE	1:100	BioLegend
	IL-13 (eBio13A)	PE-Cyanine7	1:100	eBioscience
	BCL-6 (K112–91)	FITC	1:50	BD Biosciences
	CXCR5 (L138D7)	Biotin	1:300	BioLegend
Post-fixation 2°	Streptavidin	BD Horizon™ BV421	1:400	BD Biosciences

All dilutions, except those in parentheses, represent final concentrations for staining. In the steps that require serial dilution of the antibodies, the dilutions for preparing the antibody cocktail are listed in parentheses.