. Author manuscript; available in PMC: 2022 Feb 1.

Published in final edited form as: J Allergy Clin Immunol. 2020 Jul 21;147(2):470–483. doi: 10.1016/j.jaci.2020.04.063

Table 5.

Antibody panel for peanut-specific human Tfh13 cells

Step	Antigen (Clone)	Fluorophore	Dilution^*	Source
Live/Dead	LIVE/DEAD™ Fixable Aqua Dead Cell Stain	(AmCyan channel)	1:1000	Life Technologies
Surface	CD3 (SK7)	APC-eFluor 780	1:100 (1:50)	eBioscience
	CD4 (OKT4)	Brilliant Violet 605™	1:100 (1:50)	BioLegend
	CXCR5 (RF8B2)	Alexa Fluor® 488	1:40 (1:20)	BD Biosciences
Post-fixation	CD154 (24–31)	PE	1:209 (1:9)	eBioscience
	IL-4 (MP4–25D2)	Alexa Fluor® 647	1:104.5 (1:4.5)	BioLegend
	IL-13 (JES10–5A2)	BD Horizon™ V450	1:104.5 (1:4.5)	BD Biosciences

All dilutions, except those in parentheses, represent final concentrations for staining. In the steps that require serial dilution of the antibodies, the dilutions for preparing the antibody cocktail are listed in parentheses.