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. Author manuscript; available in PMC: 2021 Aug 20.
Published in final edited form as: Cancer Gene Ther. 2020 Jul 20;28(7-8):799–812. doi: 10.1038/s41417-020-0196-5

Figure 4.

Figure 4.

Evaluation of surface binding by anti-SSTR2 mAb. A, Live-cell CLSM dynamic imaging of anti-SSTR2 mAb. Two-color CLSM: whole cell labeled with GFP (displayed as blue) and SSTR2 mAb-MMAE labeled with AF647 (red). Scale bar equals 5 μm. B, Flow cytometry to analyze the surface binding of anti-SSTR2 mAb to NET cell (BON-1) and negative control cell (917). Stained with 1 μg of mAb-AF647/million cells on ice for 30 mins. C, Western blotting of human NET (BON) xenografted tissue and mouse MTC tissues (n = 3–4) using our mAb.