Figure 3.

Metabolic difference between early and late blood collection. To identify metabolite level difference related to AaBC, 414,869 screen-negative term infants (37–41 weeks) with BW range of 2,500 g to 4,000 g and without TPN were grouped into early (12–23 h), late (49–168 h) and standard (24–48 h) collection groups. Effect size differences (Cohen's d) for each of 41 MS/MS metabolites were calculated between the early and the standard group (A) and between the late and standard group (B). In comparison to the standard, positive Cohen's d values indicate relatively higher metabolite levels in the early and the late collection groups, respectively. Metabolites are ranked from top to bottom based on Cohen's d values with primary NBS disease markers in bold.