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. 2021 Jan 20;11:601639. doi: 10.3389/fimmu.2020.601639

Figure 12.

Figure 12

Human retinal microvascular endothelial cells (HRMECs) express CXCL16, CXCR6, CX3CL1, and CX3CR1. HRMECs were left untreated or treated with interleukin-1 beta (IL-1β) (50 ng/ml) or tumor necrosis factor-alpha (TNF-α) (50 ng/ml) for 24 h. Protein expression of CXCL16 (A), CXCR6 (B), CX3CL1 (C), and CX3CR1 (D) in cell lysates was determined by Western blot analysis (representative Western blots are depicted on top of the graphs). The same loading control (β-actin) was used for quantitation of the relative band intensity of both CXCL16 and CXCR6. Levels of CX3CL1 were quantified in the culture media by ELISA (E). The box plots (median and interquartile range) show results from three different experiments performed in triplicate. Kruskal-Wallis test and Mann-Whitney tests were used for comparisons between three groups and two groups, respectively. *P < 0.05 compared with values obtained from untreated cells. #p < 0.05 compared with IL-1β-treated cells.