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. 2021 Jan 20;11:601639. doi: 10.3389/fimmu.2020.601639

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Copyright © 2021 Abu El-Asrar, Nawaz, Ahmad, De Zutter, Siddiquei, Blanter, Allegaert, Gikandi, De Hertogh, Van Damme, Opdenakker and Struyf

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot analysis of rat retinas. Rats received intravitreal injection of human CXCL16 (5 ng/5 μl) in the right eye and phosphate-buffered saline (PBS) in the left eye. The expression levels of phospho-ERK1/2 (A), the p65 subunit of NF-κB (B), intercellular adhesion molecule-1 (ICAM-1) (C) and vascular endothelial growth factor (VEGF) (D) were assessed by Western blot analysis after 24 h (A, B) or 4 days (C, D). Results are expressed as mean ± standard deviation. *p < 0.05 (independent t-test) compared to the values obtained from PBS-injected eyes.