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. Author manuscript; available in PMC: 2021 Mar 14.
Published in final edited form as: Nat Chem Biol. 2020 Sep 14;17(2):169–177. doi: 10.1038/s41589-020-0640-8

Figure 1. O-GlcNAc controls the sensitivity of fibroblasts to sphingosine-1-phosphate (S1P) mediated cell contraction.

Figure 1.

a) O-GlcNAcylation is the dynamic addition to N-acetylglucosamine to serine and threonine residues of intracellular proteins. b) O-GlcNAcylation has its own functions but can also antagonize phosphorylation, both directly and remotely, as well as combine with phosphorylation to illicit biological outcomes. c) NIH3T3 cells were treated with the indicated combinations of DMSO, the OGT inhibitor 5SGlcNAc, and/or 10% FCS. The contraction phenotype was then visualized using bright-field microscopy. d) NIH3T3 cells were treated with the indicated combinations of DMSO, 5SGlcNAc, and/or S1P. The contraction phenotype was then visualized using bright-field microscopy. The data in c and d is representative of at least two biological replicates.