Extended Data Figure 4. Inhibition of Rho kinase (ROCK1/2) blocks S1P-mediated cell contraction.

NIH3T3 cells were treated with either DMSO or 5SGlcNAc. The same cells were then treated with either additional DMSO or the ROCK1/2 inhibitor Y27632 followed by the indicated concentrations of S1P. The contraction phenotype was then visualized using bright-field microscopy. The results were then quantified and are presented as mean ± SEM of the relative culture plate area taken-up by cells in four randomly selected frames.