Fig. 3.

Comparative analyses of energy expenditure in WT and P2rx7 KO mice. a Ablation of P2X7 protein in adipose tissues (iBAT, interscapular BAT; iWAT, inguinal WAT; gWAT, gonadal WAT) of male WT and P2rx7 KO mice on the BALB/c and C57BL/6 backgrounds was confirmed by Western blotting. b–g Systematic energy expenditure in obese WT and P2rx7 KO mice on the BALB/c and C57BL/6 backgrounds were determined by indirect calorimetry. Male WT and P2rx7 KO mice were kept in metabolic monitoring cages for 1 week with a gradual decrease in housing temperature from 30 to 6 °C. O₂ consumption, CO₂ production, and respiratory exchange ratio (RER) were determined in b–d BALB/c and e–g C57BL/6 mice. For all experiments, n = 6 mice per group. Data are presented as mean ± SEM, *P < 0.05