Figure 4.

AAA lesion EOS are essential source of IL4 and EOS block NF-κB activation in macrophages, SMCs, and ECs from mouse AAA lesions. A. ELISA analysis of plasma IL4 in Apoe^–/– and Apoe^–/–ΔdblGATA mice after Ang-II perfusion-induced AAA. RT-PCR (B), immunohistology (C), and immunoblot (D) detected lesion IL4 and mEar1 in these mice. Immunofluorescent double staining of p65 detected p65 accumulation in Mac-2⁺ macrophage (E), α-actin⁺ SMC (F), and CD31⁺ EC (G) nuclei in AAA lesions from Apoe^–/– and Apoe^–/–ΔdblGATA mice at 28 days after Ang-II perfusion. Scale: 200 μm, inset: 70 μm. The number and genotype of each cohort of mice are indicated. Immunoblot analysis of p65 in cytoplasm and nucleus of BMDM (H), SMC (I), and EC (J) from Apoe^–/– and Apoe^–/–ΔdblGATA mice after cells were treated with or without TNF-α for 15 min. The numbers of experiments are indicated. Data are mean±SEM. *P<0.05, **P<0.01, ***P<0.001, Mann-Whitney U test (A-G) and one-way ANOVA test (H-J).