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. 2021 Jan 20;7:615293. doi: 10.3389/fvets.2020.615293

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Copyright © 2021 Ghosh and Malik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic analysis of the picobirnavirus (PBV) and PBV-like RNA-dependent RNA polymerase (RdRp) sequences. The phylogenetic tree was constructed by the Maximum Likelihood method using the MEGA6 software. Phylogenetic distances were measured using the LG + G model of substitution. The tree was statistically supported by bootstrapping with 500 replicates. Bootstrap values <70% are not shown. Scale bar, 0.5 substitutions per amino acid. The name of the PBV strain includes virus/host of detection/country/common name/date of collection. GenBank accession numbers are shown in parentheses. Pink circle: prototype PBV genogroup-I (GI) strain; green circle: prototype PBV GII strain; purple triangles: PBV-like viruses that use an alternative mitochondrial genetic code to translate the RdRp and cluster separately from PBVs using the standard genetic code; red triangles: PBV-like strains that use an alternative mitochondrial genetic code to translate the RdRp, yet cluster within PBVs using standard genetic code.