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Low donor density leads to a high conjugation rate. In x-axis, high density is a 1:10 dilution to the overnight donor culture and low density are 1:10,000,000 and 1:100 to an overnight donor culture of FA2-2 (pMG2200) and OG1RF (pCF10), respectively (***indicates p < 0.001, in comparison to the case with high cell density).
