KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
AAV1-CB7-mCherry	Penn Vector Core	AV-1-PV1969
AAV1-hSyn-EGFP	Penn Vector Core	AV-1-PV1696
AAV1-CAG-FLEX-tdTomato	Penn Vector Core	AV-1-ALL864
AAV9-CAG-FLEX-EFGP	Penn Vector Core	AV-9-ALL854
AAV1-hSyn-hChR2-eYFP	Penn Vector Core	AV-1–26973P
AAV1-CAMKIIa-hChR2-mcherry	Penn Vector Core	AV-1–26975
AAV1-EF1a-DIO-hChR2-eYFP	Penn Vector Core	AV-1–20298P
AAV1-EF1a-dflox-hChR2-mCherry	Addgene	20297-AAV1
AAV9-CAG-DIO-ChronosM140E-ST-p2A-H2B-mRuby	Addgene	108912- AAV9
AAV9-FLEX-ArchT-GFP	UNC Vector Core	N/A
AAV1-EF1a-FLEX-TVA-Cherry	UNC Vector Core	N/A
AAV9-CAG-FLEX-oG	Salk	N/A
SAD∆G-GFP(EnvA)	Salk	N/A
Chemicals, Peptides, and Recombinant Proteins
Alexa Fluor 488	Thermo Fisher	Cat# A10436
Alexa Fluor 594	Thermo Fisher	Cat# A10438
Red retrobeads	Lumafluor	Cat# R170
Cholera toxin subunit B- 647	Thermo Fisher	Cat# C34778
Biocytin	Sigma-Aldrich	Cat# B1758
Streptavidin- 647	Invitrogen	Cat# S21374
Fluo-5F	Invitrogen	Cat# F14221
CPP	Tocris	Cat# 0247
ZD-7288	Tocris	Cat# 1000
TTX	Tocris	Cat# 1069
4-AP	Tocris	Cat# 0940
NBQX	Tocris	Cat# 1044
Gabazine	Tocris	Cat# 1262
TEA	Tocris	Cat# 3068
QX-314	Tocris	Cat# 2313
CGP	Tocris	Cat# 1248
ProLong Gold with DAPI	Invitrogen	Cat# P36931
VectaShield with DAPI	Vector Labs	RRID: AB_2336790
Experimental Models: Organisms/Strains
Mouse/C57BL/6J	Jackson Lab	RRID: IMSR_JAX:000664
Mouse/NDNF-Cre	Jackson Lab	RRID: IMSR_JAX:028536
Mouse/Ai14	Jackson Lab	RRID: IMSR_JAX:007908
Mouse/VIP-Cre	Jackson Lab	RRID: IMSR_JAX: 010908
Mouse/G42	Jackson Lab	RRID: IMSR_JAX: 007677
Mouse/GIN	Jackson Lab	RRID: IMSR_JAX: 003718
Mouse/5HT3aR-Cre	GENSAT	RRID: MMRRC_036680-UCD
Software and Algorithms
Igor Pro	WaveMetrics	RRID: SCR_000325
MATLAB	MathWorks	RRID: SCR_001622
Prism	GraphPad Software	RRID: SCR_002798
Neurolucida 360	MBF Bioscience	RRID: SCR_001775